Analysis of DNA polymerase activity in vitro using non-radioactive primer extension assay in an automated DNA sequencer.

@article{Lopes2007AnalysisOD,
  title={Analysis of DNA polymerase activity in vitro using non-radioactive primer extension assay in an automated DNA sequencer.},
  author={D{\'e}bora O Lopes and Carlos Gustavo R{\'e}gis-da-Silva and Alice Machado-Silva and Andr{\'e}a Mara Macedo and Gl{\'o}ria R. Franco and Jean S{\'e}bastien Hoffmann and Christophe Cazaux and S{\'e}rgio D. Junho Pena and Santuza Maria Ribeiro Teixeira and Carlos Renato Machado},
  journal={Genetics and molecular research : GMR},
  year={2007},
  volume={6 2},
  pages={250-5}
}
Although different DNA polymerases have distinct functions and substrate affinities, their general mechanism of action is similar. Thus, they can all be studied using the same technical principle, the primer extension assay employing radioactive tags. Even though fluorescence has been used routinely for many years for DNA sequencing, it has not been used in the in vitro primer extension assay. The use of fluorescence labels has obvious advantages over radioactivity, including safety, speed and… CONTINUE READING