Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction.

  title={Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction.},
  author={Beverly Barton Rogers and Lesley C. Alpert and E A Hine and Gregory J. Buffone},
  journal={The American journal of pathology},
  volume={136 3},
The polymerase chain reaction (PCR) was used to amplify viral or oncogene sequences from frozen or formalin-fixed, paraffin-embedded tissue sections. Methods for preparing fixed, embedded colonic tissue for PCR amplification of c-K-ras sequences from genomic DNA and for amplification of viral DNA from other tissues, including brain, lung, and liver, were evaluated. The effect of formalin fixation on the efficiency of amplification was also determined. While there seemed to be only a modest… CONTINUE READING


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