Analysis of DNA double strand breakage and repair using orthogonal field alternation gel electrophoresis.

@article{Contopoulou1987AnalysisOD,
  title={Analysis of DNA double strand breakage and repair using orthogonal field alternation gel electrophoresis.},
  author={C R Contopoulou and V E Cook and Robert K. Mortimer},
  journal={Yeast},
  year={1987},
  volume={3 2},
  pages={71-6}
}
Orthogonal field alternation gel electrophoresis (OFAGE) allows separation of DNA molecules in the size range of 200 kb to 3000 kb. These sizes encompass the chromosome sizes of the genome of Saccharomyces cerevisiae. Using this technique, we have found that yeast cells exposed to X-rays generate a smear of DNA fragments corresponding to the products of random, independent double strand breaks, and that the bands corresponding to unbroken chromosomes decrease in intensity in direct proportion… CONTINUE READING