Analysis of DNA-RecA protein interactions involving the protein self-association reaction.

@article{Takahashi1989AnalysisOD,
  title={Analysis of DNA-RecA protein interactions involving the protein self-association reaction.},
  author={Masayuki Takahashi},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 1},
  pages={288-95}
}
A method to analyze the DNA binding properties of RecA protein is developed to take into account the protein self-association reaction and is applied to reanalyze the interaction with chemically modified single-stranded DNA (epsilon-DNA). Protein oligomerization is investigated by static light-scattering measurements and analyzed with the accumulated strain model. A coupled equilibrium between DNA binding and self-association of RecA is resolved considering that the complex formed between an m… CONTINUE READING

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