Analysis of Corneal Aldehyde Dehydrogenase Patterns in Pathologic Corneas

  title={Analysis of Corneal Aldehyde Dehydrogenase Patterns in Pathologic Corneas},
  author={Tjahyono D. Gondhowiardjo and Nicolaas J. van Haeringen and Hennie J. M. V{\"o}lker‐Dieben and Houdijn W. Beekhuis and Jan H. C. Kok and Gabriel van Rij and Liesbeth Pels and Aize Kijlstra},
In this study we investigated the properties of corneal aldehyde dehydrogenase (ALDH) in keratoconus corneas using various electrophoretic techniques combined with immunochemical and zymographic identification. Normal corneas and other pathologic corneal buttons obtained during keratoplastic surgery were used as a control. A significant (p<0.001) lower enzymatic activity was found in keratoconus epithelial extracts (3.1 ± 2.1 IU/mg protein) compared with normal controls (5.5 ± 2.6 IU/mg protein… Expand
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