Analysis of Corneal Aldehyde Dehydrogenase Patterns in Pathologic Corneas

@article{Gondhowiardjo1993AnalysisOC,
  title={Analysis of Corneal Aldehyde Dehydrogenase Patterns in Pathologic Corneas},
  author={Tjahyono D. Gondhowiardjo and Nicolaas J. van Haeringen and Hennie J. M. V{\"o}lker‐Dieben and Houdijn W. Beekhuis and Jan H. C. Kok and Gabriel van Rij and Liesbeth Pels and Aize Kijlstra},
  journal={Cornea},
  year={1993},
  volume={12},
  pages={146-154}
}
In this study we investigated the properties of corneal aldehyde dehydrogenase (ALDH) in keratoconus corneas using various electrophoretic techniques combined with immunochemical and zymographic identification. Normal corneas and other pathologic corneal buttons obtained during keratoplastic surgery were used as a control. A significant (p<0.001) lower enzymatic activity was found in keratoconus epithelial extracts (3.1 ± 2.1 IU/mg protein) compared with normal controls (5.5 ± 2.6 IU/mg protein… Expand
Marked reduction of alcohol dehydrogenase in keratoconus corneal fibroblasts
TLDR
Cutting alcohol dehydrogenase in KC corneal fibroblasts represents a strong marker and possible mediator of keratoconus and is confirmed by western blot analysis of early passage primary keratocyte cell lysates. Expand
Investigation of Heme Oxygenase 2 Enzyme Protein Expression in Keratoconus and Normal Human Corneal Epithelium: An Immunohistochemical Study
TLDR
The HO-2 enzyme staining using immunohistochemical methods was at lower amounts in the keratoconic corneal epithelial cells as compared with normal corneals, suggesting that this enzyme may play a role in the etiopathogenesis of keratoconus. Expand
Role of biochemical factors in the pathogenesis of keratoconus.
Keratoconus (KC) is a corneal disease associated with structural abnormalities in the corneal epithelium, Bowman's layer and stroma and altered concentration of tear components. KC corneas show aExpand
Oxidative stress in keratoconus?
TLDR
The increased levels of oxidative stress markers and the decreased antioxidant capacity and antioxidant defenses in KC corneas, as well as in the post-LASIK ectatic cornea, indicate that oxidative stress might be involved in the development of this disease and may provide new insights for its prevention and treatment in the future. Expand
Differential epithelial and stromal protein profiles in keratoconus and normal human corneas.
TLDR
The results suggested that the Nano-ESI-LC-MS(MS)(2) method was superior than the 2D-DIGE method as it identified a greater number of proteins with altered levels in KC corneas. Expand
Alkali burn causes aldehyde dehydrogenase 3A1 (ALDH3A1) decrease in mouse cornea.
TLDR
Alkali burn of the corneal surface caused a rapid decrease of ALDH3A1 in the cornea at both the RNA and protein levels, which leads to the loses of the protective component of the CORNEal surface and makes it vulnerable to further damage. Expand
Corneal aldehyde dehydrogenase and glutathione S-transferase activity after excimer laser keratectomy in guinea pigs
TLDR
The results suggest that excimer laser treatment of high myopia may change the ALDH and GST activities, metabolism, and free radical balance of the cornea. Expand
Evidence of Oxidative Stress in Human Corneal Diseases
TLDR
The results suggest that keratoconus corneas do not process reactive oxygen species in a normal manner, which may play a major role in the pathogenesis of this disease. Expand
Corneal aldehyde dehydrogenases: Multiple functions and novel nuclear localization
TLDR
Examination of gene expression of other ALDHs found high messenger levels of ALDH1B1, ALDH2 and ALDH7A1 in mouse cornea and lens and shows that ALDH3A1 is also found in the nucleus of rabbit keratocytes, suggesting the nuclear presence of AL DH3A 1 may be involved in cell cycle regulation. Expand
Ocular aldehyde dehydrogenases: Protection against ultraviolet damage and maintenance of transparency for vision
TLDR
As a result of their catalytic and non-catalytic functions, ALDH3A1 and ALDH1A1 proteins protect inner ocular tissues from ultraviolet radiation and reactive oxygen-induced damage and contribute to cellular transparency in corneal stromal keratocytes, supporting a structural role of these ALDH proteins. Expand
...
1
2
3
4
5
...