Analysis of Complex Protein Mixtures Using Nano-LC Coupled to MS/MS.

INTRODUCTIONNanoliter-LC coupled to tandem mass spectrometry (nano-LC-MS/MS) permits the rapid and sensitive determination of protein-protein interactions. By using a specific purification technique such as coimmunoprecipitation or affinity purification in conjunction with nano-LC-MS/MS, not only are proteins identified, but specific protein-protein… CONTINUE READING