Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins.

@article{Varsaki2009AnalysisOC,
  title={Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins.},
  author={Athanasia Varsaki and Gabriel Moncali{\'a}n and Maria del Pilar Garcill{\'a}n-Barcia and Constantin Drainas and F Sebasti{\'a}n de la Cruz},
  journal={Journal of bacteriology},
  year={2009},
  volume={191 5},
  pages={1446-55}
}
MbeC is a 13-kDa ColE1-encoded protein required for efficient mobilization of ColE1, a plasmid widely used in cloning vector technology. MbeC protein was purified and used for in vitro DNA binding, which showed that it binds specifically double-stranded DNA (dsDNA) containing the ColE1 oriT. Amino acid sequence comparison and secondary structure prediction imply that MbeC is related to the ribbon-helix-helix (RHH) protein family. Alignment with RHH members pointed to a conserved arginine (R13… CONTINUE READING
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