Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides.

@article{Kern2000AnalysisOC,
  title={Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides.},
  author={Florian G. Kern and Nicole Faulhaber and Cornelius Froemmel and Elham Khatamzas and Susanna Proesch and Constanze Schoenemann and Ines Kretzschmar and Rudolf Volkmer-Engert and H. D. Volk and Petra Reinke},
  journal={European journal of immunology},
  year={2000},
  volume={30 6},
  pages={1676-82}
}
The frequencies of human cytomegalovirus (HCMV) protein-specific CD8 T cells, identified by the presence of intracellular IFN-gamma, were measured by flow cytometry following stimulation of freshly isolated peripheral blood mononuclear cells (PBMC) with comprehensive peptide pools. These pools spanned the entire amino acid sequences of the HCMV pp65 and major immediate early (IE-1) proteins and consisted of 15-amino acid peptides with at least nine overlaps between neighboring peptides. As a… CONTINUE READING

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