Analysis of C5a-mediated chemotaxis by lentiviral delivery of small interfering RNA.

@article{Hwang2004AnalysisOC,
  title={Analysis of C5a-mediated chemotaxis by lentiviral delivery of small interfering RNA.},
  author={Jong-Ik Hwang and Iain D. C. Fraser and Sangdun Choi and X Qin and Melvin I. Simon},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 2},
  pages={488-93}
}
Immune cells respond to chemotactic signals by means of G protein-coupled receptors. Attempts to elucidate the function of specific G protein family members in these responses is complicated by redundancy among the different G protein isoforms. We have used lentiviral-based RNA interference to eliminate expression of specific G protein subunits selectively in J774A.1 mouse macrophages. The chemotactic response to the complement factors C5a and C3a is ablated in cells lacking G beta(2) but is… CONTINUE READING

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