Analysis of B-cell activation of cerebrospinal fluid lymphocytes in multiple sclerosis.

@article{Levinson1983AnalysisOB,
  title={Analysis of B-cell activation of cerebrospinal fluid lymphocytes in multiple sclerosis.},
  author={Arnold I. Levinson and Magnhild Sandberg-Wollheim and Robert P. Lisak and Burton Zweiman and K Sjogren and C Laramore and A R Moskovitz and S A Hedstrom},
  journal={Neurology},
  year={1983},
  volume={33 10},
  pages={1305-10}
}
We have developed a microculture system to study pokeweed mitogen (PWM)-induced B-cell differentiation responses of CSF lymphocytes from patients with multiple sclerosis (MS) and other neurologic diseases (OND). B-cell differentiation was assessed by (1) enumeration of immunoglobulin-secreting cells (IgSC) by a protein A reverse hemolytic plaque assay; and (2) quantitation of supernatant IgG by ELISA. Cultures of MS CSF cells and OND CSF cells responded to PWM with a similar frequency, with… CONTINUE READING