Analysis of B-6 vitamers in human milk by reverse-phase liquid chromatography.

@article{Hamaker1985AnalysisOB,
  title={Analysis of B-6 vitamers in human milk by reverse-phase liquid chromatography.},
  author={Bruce R Hamaker and Avanelle Kirksey and A Ekanayake and Marlene W Borschel},
  journal={The American journal of clinical nutrition},
  year={1985},
  volume={42 4},
  pages={650-5}
}
A simple, accurate reverse-phase high performance liquid chromatography (RPLC) method was introduced for the analysis of B-6 vitamers in human milk. The assay consisted of a phosphate buffer (pH 2.9) delivered isocratically through 5 micron ODS column packing, followed by post-column bisulfite derivatization to enhance the detection of PLP (and to a minor extent PL). The vitamers were detected using a fluorescence spectromonitor. Sample run time was less than 30 min. The sensitivity of the… CONTINUE READING