Analysis of Australian fur seal diet by pyrosequencing prey DNA in faeces.

  title={Analysis of Australian fur seal diet by pyrosequencing prey DNA in faeces.},
  author={Bruce E Deagle and Roger Kirkwood and Simon N. Jarman},
  journal={Molecular ecology},
  volume={18 9},
DNA-based techniques have proven useful for defining trophic links in a variety of ecosystems and recently developed sequencing technologies provide new opportunities for dietary studies. We investigated the diet of Australian fur seals (Arctocephalus pusillus doriferus) by pyrosequencing prey DNA from faeces collected at three breeding colonies across the seals' range. DNA from 270 faecal samples was amplified with four polymerase chain reaction primer sets and a blocking primer was used to… CONTINUE READING
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