Analysis of Aleutian disease of mink parvovirus infection using strand-specific hybridization probes.

@article{Bloom1987AnalysisOA,
  title={Analysis of Aleutian disease of mink parvovirus infection using strand-specific hybridization probes.},
  author={Marshall E. Bloom and Richard E. Race and James B. Wolfinbarger},
  journal={Intervirology},
  year={1987},
  volume={27 2},
  pages={102-11}
}
A 7- to 95-map unit segment of DNA from Aleutian disease of mink parvovirus (ADV) was subcloned into a bacteriophage SP6 based transcription vector and used to produce radiolabeled viral RNA transcripts corresponding to either 'plus' or 'minus' sense. The radiolabeled transcripts were reacted against Southern blots of whole cell DNA from ADV infected cell cultures as hybridization probes. The 'plus' sense RNA probe hybridized both to duplex replicative forms (RFs) as well as to single-stranded… CONTINUE READING

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