Analysis of ALV-based packaging cell lines for production of contaminant defective viruses.

@article{Girod1995AnalysisOA,
  title={Analysis of ALV-based packaging cell lines for production of contaminant defective viruses.},
  author={Anne Girod and Francois Loic Cosset and G{\'e}rard Verdier and Corinne Ronfort},
  journal={Virology},
  year={1995},
  volume={209 2},
  pages={671-5}
}
We have previously described avian leukosis virus-based packaging cell lines that express gag, pol, and env proteins from two transcomplementing genomes and produce helper-free stocks of retroviral vectors with different host ranges. In this report, we demonstrated that (i) despite the deletion of the psi packaging sequence, the packaging-defective transcomplementing retroviral transcripts were packaged into virions at a level that could reach 2.3% of a wild-type virus packaging level and (ii… CONTINUE READING