Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography.


A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2-carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reverse-phase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability, and excellent chromatographic separation and… (More)


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