Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.

@article{Steiger2005AnalysisO2,
  title={Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.},
  author={Michelle A Steiger and Jane E. Jackman and Eric M Phizicky},
  journal={RNA},
  year={2005},
  volume={11 1},
  pages={99-106}
}
Tpt1p is an essential protein responsible for the 2'-phosphotransferase step of tRNA splicing in Saccharomyces cerevisiae, in which the splice junction 2'-phosphate of ligated tRNA is transferred to NAD to form mature tRNA and ADP-ribose 1''-2'' cyclic phosphate. We showed previously that Tpt1p is a member of a family of functional 2'-phosphotransferases… CONTINUE READING