Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria.

@article{Salzman2002AnalysisO1,
  title={Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria.},
  author={Nita H. Salzman and Hendrik de Jong and Yvonne Paterson and Hermie J. M. Harmsen and Gjalt W. Welling and Nicolaas A. Bos},
  journal={Microbiology},
  year={2002},
  volume={148 Pt 11},
  pages={3651-60}
}
Total genomic DNA from samples of intact mouse small intestine, large intestine, caecum and faeces was used as template for PCR amplification of 16S rRNA gene sequences with conserved bacterial primers. Phylogenetic analysis of the amplification products revealed 40 unique 16S rDNA sequences. Of these sequences, 25% (10/40) corresponded to described intestinal organisms of the mouse, including Lactobacillus spp., Helicobacter spp., segmented filamentous bacteria and members of the altered… CONTINUE READING