Analysis for alpha-amylase isoenzymes by automated isoelectric focusing.


We describe the fractionation of alpha-amylase (EC isoenzymes by use of the Pharmacia "Phastsystem" electrophoresis apparatus. The separation is done by isoelectric focusing on "Phastgel IEF 5-8." A complete run, including staining, takes 4 h and can easily resolve eight isoenzymes. Samples can be analyzed in a routine laboratory with use of… (More)


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