Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl-tRNA synthetase.

Abstract

We have cloned and sequenced the Staphylococcus aureus Oxford ileS gene which encodes isoleucyl-tRNA synthetase (Ile-RS), the target for the antibiotic mupirocin. The gene was identified by hybridisation to oligodeoxyribonucleotide probes derived from internal Ile-RS amino acid (aa) sequences. The 2754-bp open reading frame encodes a 918-aa protein of 105… (More)

Topics

Cite this paper

@article{Chalker1994AnalysisAT, title={Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl-tRNA synthetase.}, author={Alan Chalker and J. M. Ward and Andrew P. Fosberry and J. P. E. Hodgson}, journal={Gene}, year={1994}, volume={141 1}, pages={103-8} }