Analysis and characterization of the expression of the secretory and membrane forms of IgM heavy chains in the pufferfish, Takifugu rubripes.

@article{Saha2005AnalysisAC,
  title={Analysis and characterization of the expression of the secretory and membrane forms of IgM heavy chains in the pufferfish, Takifugu rubripes.},
  author={Nil Ratan Saha and Hiroaki Suetake and Yuzuru Suzuki},
  journal={Molecular immunology},
  year={2005},
  volume={42 1},
  pages={113-24}
}
We investigated the structure and expression of immunoglobulin genes in the pufferfish, Takifugu rubripes, a highly prized and economically important fish species. The cDNA fragment that partially encodes the constant region of the IgM heavy chain was isolated in these animals by RACE using degenerate primers after which it was used as a probe for screening IgM heavy chains in a fugu splenic cDNA library. The structural feature of the constant region of fugu sIgM was found to consist of four… CONTINUE READING

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