Analysing traces of autoinducer-2 requires standardization of the Vibrio harveyi bioassay.

@article{Vlchez2007AnalysingTO,
  title={Analysing traces of autoinducer-2 requires standardization of the Vibrio harveyi bioassay.},
  author={Ramiro V{\'i}lchez and Andr{\'e} Lemme and Verena Thiel and Stefan Schulz and Helena Sztajer and Irene Wagner-D{\"o}bler},
  journal={Analytical and bioanalytical chemistry},
  year={2007},
  volume={387 2},
  pages={489-96}
}
Autoinducer-2 (furanosyl borate diester) is a biologically active compound whose role as a universal bacterial signalling molecule is currently under intense investigation. Because of its instability and the low concentrations of it found in biological samples, its detection relies at present on a bioassay that measures the difference in the timing of the luminescence of the Vibrio harveyi BB170 sensor strain with and without externally added AI-2. Here we systematically investigated which… CONTINUE READING

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