Analysing DNA methylation using bisulphite pyrosequencing.


Bisulphite pyrosequencing is a quantitative methodology for the investigation of DNA methylation of sequences up to 100-bp in length. Biotin-labelled, single-stranded PCR products generated from bisulphite-treated DNA are used as a template with an internal primer to perform the pyrosequencing reaction. Nucleotides are added in a predetermined order in each… (More)
DOI: 10.1007/978-1-61779-316-5_4


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