Analyses of protein-protein interactions by in vivo photocrosslinking in budding yeast.


Recent development of methods for genetic incorporation of unnatural amino acids into proteins in live cells enables us to analyze protein interactions by site-specific photocrosslinking. Here we describe a method to incorporate p-benzoyl-L-phenylalanine (pBpa), a photoreactive unnatural amino acid, into defined positions of a target protein in living yeast… (More)
DOI: 10.1007/978-1-62703-487-6_14


  • Presentations referencing similar topics