Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma

@article{Robert1999AnalysesIT,
  title={Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma},
  author={Laurian S. Robert and Madeleine L{\'e}vesque-Lemay and Jean L. Gerster and H. P. Hong and Wilf Keller},
  journal={Plant Cell Reports},
  year={1999},
  volume={18},
  pages={357-362}
}
A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme β-glucuronidase (GUS) and introduced into tobacco via Agrobacterium-mediated transformation. The promoter fragment directed expression of… CONTINUE READING