Anaerobic degradation of benzoate to methane by a microbial consortium

  title={Anaerobic degradation of benzoate to methane by a microbial consortium},
  author={James G. Ferry and Ralph S. Wolfe},
  journal={Archives of Microbiology},
  • J. Ferry, R. Wolfe
  • Published 1 February 1976
  • Chemistry, Medicine
  • Archives of Microbiology
A stabilized consortium of microbes which anaerobically degraded benzoate and produced CH4 was established by inoculation of a benzoate-mineral salts medium with sewage sludge; the consortium was routinely subcultured anaerobically in this medium for 3 years. Acetate, formate, H2 and CO2 were identified as intermediates in the overall conversion of benzoate to CH4 by the culture. Radioactivity was equally divided between the CH4 and CO2 from the degradation of uniformly ring-labeled [14C… 
Isolation and characterization of an anaerobic syntrophic benzoate-degrading bacterium from sewage sludge
An anaerobic, motile, gram-negative, rod-shaped bacterium is described which degrades benzoate in coculture with an H2-utilizing organism and in the absence of exogenous electron acceptors such as
Degradation of phenol by a bacterial consortium under methanogenic conditions
An anaerobic bacterial consortium was shown to carboxylate phenol to benzoate under methanogenic conditions. Benzoate accumulated in the culture medium and was completely degraded when the incubation
Anaerobic degradation of phenol in sewage sludge
Anaerobic phenol degrading consortia were selected in sewage sludge and culture conditions were improved to allow maximum degradation rates of 0.9 g/l·d, leading to a reduction of the phenol degradation rates and presumably to an increased concentration of benzoate.
Interspecies acetate transfer influences the extent of anaerobic benzoate degradation by syntrophic consortia.
A critical or minimal Gibb's free energy may exist where thermodynamic constraints preclude further benzoate degradation in cell suspensions with a threshold and in suspensions without a threshold.
Methane Production from Glucose by a Mixed Culture of Bacteria in the Chemostat : the Role of Citrobacter
Summary: A chemostat culture under a nitrogen atmosphere with a formate and inorganic salts medium fed at dilution rates up to 0·1 h-1 and inoculated from a sewage works anaerobic digester, proved to
Methanogenic Degradation of Sodium Benzoate in Profundal Sediments from a Small Eutrophic Lake
SUMMARY: Enrichments were established to examine the potential of Blelham Tarn profundal sediment to metabolize benzoate to CH4 and CO2. Long‘adaptation’ times were required before benzoate-dependent
Transformation of indole by methanogenic and sulfate-reducing microorganisms isolated from digested sludge
The investigation demonstrates that indole can be transformed by both methanogenic and sulfate-reducing microbial populations and is transformed to methane and carbon dioxide.
Fermentative degradation of resorcinol and resorcylic acids
Anaerobic fermentative degradation of resorcinol and resorcylates was studied in enrichment cultures inoculated with marine or freshwater sediments or digested sludge, andhibition of methanogenic bacteria by bromoethanesulfonate and acetylene led to enhanced acetate formation indicating homoacetogenic hydrogen oxidation.
Methane formation from fructose by syntrophic associations of Acetobacterium woodii and different strains of methanogens
When Acetobacterium woodii was co-cultured in continuous or in stationary culture with Methanobacterium strain AZ, fructose instead of being converted to 3 mol of acetate was converted to 2 mol of
Energy conservation mechanisms and electron transfer in syntrophic propionate-oxidizing microbial consortia
Syntrophic methanogenic associations between acetogenic bacteria and methanogenic archaea are essential for the complete mineralization of organic compounds to methane and CO2. Propionate and


Anaerobic Degradation of the Benzene Nucleus by a Facultatively Anaerobic Microorganism
The patterns of the oxidation of aromatic substrates with oxygen or nitrate by cells grown aerobically or anaerobically on different aromatic compounds indicated that benzoate rather than protocatechuate was a key intermediate in the early stages of anaerobic metabolism.
The anaerobic degradation of aromatic compounds by a denitrifying bacterium
Summary1.Pseudomonas PN-1, grown anaerobically on a benzoate-nitrate medium, produced 14CO2 from either carboxyl-labelled or uniformly ring-labelled 14C-benzoate upon aerobic or anaerobic incubation.
Anaerobic Metabolism of Aromatic Substrates by Certain Micro-organisms
Anaerobic Metabolism of Aromatic Substrates by Certain Micro-organisms R. J. WILLIAMS and W. C. EVANS Department of Biochemistry arid Soil Science, University College of North Wales, Bangor, Caerns.,
Tracer experiments on the origin of methane are reported, carried out with another acetatefermenting bacterium, a species of Methanosarcina, to provide additional examples of fermentations wherein methane arises chiefly from the organic substrate and carbon dioxide reduction appears to be of little importance.
Microbial formation of methane.
  • R. Wolfe
  • Chemistry, Medicine
    Advances in microbial physiology
  • 1971
Fermentation of Fructose and Synthesis of Acetate from Carbon Dioxide by Clostridium formicoaceticum
It is proposed that acetate is totally synthesized from CO(2) via the reactions catalyzed by the enzymes listed above and that 5-methyltetra-hydrofolate and a methylcorrinoid are intermediates in this synthesis.
The metabolism of aromatic compounds by Rhodopseudomonas palustris. A new, reductive, method of aromatic ring metabolism.
The photometabolism of benzoate is accomplished by a novel reductive pathway involving its reduction to cyclohex- 1-ene-1-carboxylate, followed by hydration to 2-hydroxycyclohexanecarboxylates, and after dehydrogenation to2-oxocyclohexAnecar boxylate further hydration results in ring-fission and the production of pimelate.
Enzymatic phosphorylation of acetate.