Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development.

  title={Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development.},
  author={T H Wei and T. S. Ramasubramanian and James W Golden},
  journal={Journal of bacteriology},
  volume={176 15},
The Anabaena sp. strain PCC 7120 ntcA (bifA) gene encodes a sequence-specific DNA-binding protein, NtcA (BifA, VF1) that interacts with the upstream region of several genes, including glnA, xisA, rbcL, and nifH. We have constructed a ntcA null mutant by interrupting the gene with an omega Spr-Smr cassette. The ntcA mutant was not able to grow with nitrate or atmospheric dinitrogen as the sole nitrogen source but could be grown on medium containing ammonium. The ntcA mutant was unable to form… CONTINUE READING
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