An update for human blood plasma pretreatment for optimized recovery of low-molecular-mass peptides prior to CE–MS and SPE–CE–MS.

Abstract

Protein precipitation and centrifugal filtration are well-established methods for concentrating and purifying peptides with a low relative molecular mass (Mr) from human blood plasma before proteomic and peptidomic studies using high-performance separation techniques, but there is little information on peptide recoveries. Here, we evaluate acetonitrile… (More)

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