An unusual halotolerant a-type carbonic anhydrase from the alga Dunaliella salina functionally expressed in Escherichia coli

@inproceedings{Premkumar2002AnUH,
  title={An unusual halotolerant a-type carbonic anhydrase from the alga Dunaliella salina functionally expressed in Escherichia coli},
  author={Lakshmanane Premkumar and Umesh K. Bageshwar and Irena Gokhman and Ada Zamir and Joel L. Sussmanb},
  year={2002}
}
A 60-kDa, salt-inducible, internally duplicated a-type carbonic anhydrase (Dca) is associated with the plasma membrane of the extremely salt-tolerant, unicellular, green alga Dunaliella salina. Unlike other carbonic anhydrases, Dca remains active over a very broad range of salinities (0–4M NaCl), thus representing a novel type of extremely halotolerant enzyme. To elucidate the structural principles of halotolerance, structure–function investigations of Dca have been initiated. Such studies… CONTINUE READING

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