An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates

@article{Shin1985AnUC,
  title={An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates},
  author={Hee Sup Shin and Thaddeus A. Bargiello and B. Ted Clark and F Rob Jackson and Michael W. Young},
  journal={Nature},
  year={1985},
  volume={317},
  pages={445-448}
}
The per locus has a fundamental involvement in the expression of biological rhythms in Drosophila. Mutations at this locus can shorten, lengthen or eliminate a variety of rhythmic activities that range from circadian behaviours, exemplified by eclosion and locomotor activities1,2, to short-period behaviour such as the 55-s rhythm of courtship song3. DNA from the per locus has been cloned4–7, and we have used P-element-mediated DNA transformation to establish that a 7.1-kilobase (kb) HindIII… 

Product of per locus of Drosophila shares homology with proteoglycans

A fragment of DNA of ∼7 kilobases (kb) encoding a 4.5-kb poly(A)+ RNA restores rhyth-micity when transduced into Drosophila carrying mutations5,6 or chromosomal deletions5 of the per locus and the sequence of this biologically active segment of DNA is reported.

Molecular mapping of point mutations in the period gene that stop or speed up biological clocks in Drosophila melanogaster.

The pero1 and the pers mutations in Drosophila melanogaster, which seem to eliminate or speed up, respectively, the clocks underlying biological rhythmicity, were mapped to single nucleotides.

The Neurospora clock gene frequency shares a sequence element with the Drosophila clock gene period

The cloning and analysis of the frq locus are reported and show it to be larger and more complex than would have been predicted from the available genetic data, and suggests the possibility of a common element in the clock mechanisms of these two organisms.

Mutagenesis of the clock gene period in Drosophila melanogaster

The period gene is an essential component of the circadian oscillator in D.melanogaster and the removal of sequence 3’ to the repeat unexpectedly causes shortening of the period, which suggests that the Thr-Gly deletions may disrupt an interaction with another factor that plays a part in establishing locomotor activity patterns.

Changes in abundance or structure of the per gene product can alter periodicity of the Drosophila clock

It is suggested that perl and pers mutants produce hypoactive and hyperactive per proteins, respectively, which are inversely correlated with period length, so that flies with lowest levels of the per product have slow-running biological clocks.

The molecular ethology of theperiod gene inDrosophila

A model has been proposed, which suggests that the period (per) gene in Drosophila may determine periodicity by modulating intercellular communication.

The period clock locus of D. melanogaster codes for a proteoglycan

...

References

SHOWING 1-10 OF 20 REFERENCES

Molecular genetics of a biological clock in Drosophila.

  • T. BargielloM. W. Young
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
Physical characterization of a series of chromosomal rearrangements altering per locus activity indicates that DNA affecting behavioral rhythms is found in a 7.1-kb HindIII fragment.

Restoration of circadian behavioural rhythms by gene transfer in Drosophila

It is reported that when a 7.1-kb fragment from a per+ fly, including the sequences encoding the 4.5-kb transcript, is introduced into the genome of a per0 (arrhythmic) fly by P element-mediated transformation, circadian rhythmicity of behaviour such as eclosion and locomotor activity is restored.

Circadian rhythm mutations in Drosophila melanogaster affect short-term fluctuations in the male's courtship song.

  • C. KyriacouJ. C. Hall
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1980
Both circadian rhythms and a very short, noncircadian oscillation appear to be influenced by the same gene.

Nonessential Sequences, Genes, and the Polytene Chromosome Bands of DROSOPHILA MELANOGASTER.

The discovery of these units, which are not allelic to any of the loci previously known, makes it clear that division 3B contains more genes than polytene chromosome bands, while portions of 3A and 3C seem to have no functional significance.

Clock mutants of Drosophila melanogaster.

  • R. KonopkaS. Benzer
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1971
Three mutants have been isolated in which the normal 24-hour rhythm is drastically changed and all these mutations appear to involve the same functional gene on the X chromosome.