An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates

@article{Shin1985AnUC,
  title={An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates},
  author={Hee-Sup Shin and Thaddeus A. Bargiello and Brian T. Clark and F Rob Jackson and Michael W. Young},
  journal={Nature},
  year={1985},
  volume={317},
  pages={445-448}
}
The per locus has a fundamental involvement in the expression of biological rhythms in Drosophila. Mutations at this locus can shorten, lengthen or eliminate a variety of rhythmic activities that range from circadian behaviours, exemplified by eclosion and locomotor activities1,2, to short-period behaviour such as the 55-s rhythm of courtship song3. DNA from the per locus has been cloned4–7, and we have used P-element-mediated DNA transformation to establish that a 7.1-kilobase (kb) HindIII… Expand
Product of per locus of Drosophila shares homology with proteoglycans
TLDR
A fragment of DNA of ∼7 kilobases (kb) encoding a 4.5-kb poly(A)+ RNA restores rhyth-micity when transduced into Drosophila carrying mutations5,6 or chromosomal deletions5 of the per locus and the sequence of this biologically active segment of DNA is reported. Expand
Molecular mapping of point mutations in the period gene that stop or speed up biological clocks in Drosophila melanogaster.
The pero1 and the pers mutations in Drosophila melanogaster, which seem to eliminate or speed up, respectively, the clocks underlying biological rhythmicity, were mapped to single nucleotides.Expand
The Neurospora clock gene frequency shares a sequence element with the Drosophila clock gene period
TLDR
The cloning and analysis of the frq locus are reported and show it to be larger and more complex than would have been predicted from the available genetic data, and suggests the possibility of a common element in the clock mechanisms of these two organisms. Expand
Mutagenesis of the clock gene period in Drosophila melanogaster
TLDR
The period gene is an essential component of the circadian oscillator in D.melanogaster and the removal of sequence 3’ to the repeat unexpectedly causes shortening of the period, which suggests that the Thr-Gly deletions may disrupt an interaction with another factor that plays a part in establishing locomotor activity patterns. Expand
Molecular cloning of a gene under control of the circadian clock and light in the rodent SCN.
TLDR
The results suggest that the transcriptional control of mp41 gene is regulated by light and a circadian clock and indicate that mp41 is a new marker gene for a cycling transcript in the SCN. Expand
Changes in abundance or structure of the per gene product can alter periodicity of the Drosophila clock
TLDR
It is suggested that perl and pers mutants produce hypoactive and hyperactive per proteins, respectively, which are inversely correlated with period length, so that flies with lowest levels of the per product have slow-running biological clocks. Expand
Circadian rhythms in Drosophila melanogaster: analysis of period as a function of gene dosage at the per (period) locus.
TLDR
Three possible roles for the per gene product in circadian rhythmicity are discussed, including a role in synchronizing rhythm-producing cells and an explanation for the partial dominance of pers over per+ and the dominance of per+ over per1. Expand
The molecular ethology of theperiod gene inDrosophila
TLDR
A model has been proposed, which suggests that the period (per) gene in Drosophila may determine periodicity by modulating intercellular communication. Expand
The Drosophila per gene homologs are expressed in mammalian suprachiasmatic nucleus and heart as well as in molluscan eyes.
This study presents evidence for the conservation of Drosophila per gene homologs in mammalian DNA and for their expression in a number of tissues which are involved in various aspects of circadianExpand
The period clock locus of D. melanogaster codes for a proteoglycan
TLDR
To determine whether the per transcript codes for a proteoglycan, a region of its coding sequence was expressed (in bacteria) as part of a fusion protein, which was used to immunize rabbits and detected an antigen that is present in wild-type flies and absent in a per- mutant. Expand
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References

SHOWING 1-10 OF 20 REFERENCES
Molecular genetics of a biological clock in Drosophila.
  • T. Bargiello, M. W. Young
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1984
TLDR
Physical characterization of a series of chromosomal rearrangements altering per locus activity indicates that DNA affecting behavioral rhythms is found in a 7.1-kb HindIII fragment. Expand
Restoration of circadian behavioural rhythms by gene transfer in Drosophila
TLDR
It is reported that when a 7.1-kb fragment from a per+ fly, including the sequences encoding the 4.5-kb transcript, is introduced into the genome of a per0 (arrhythmic) fly by P element-mediated transformation, circadian rhythmicity of behaviour such as eclosion and locomotor activity is restored. Expand
Molecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms
TLDR
Evidence is discussed, from previously reported genetic and phenotypic analysis of per's function, suggesting that this region may be complex and that several gene products from the per region, including this 0.9 kb transcript, may be involved in the different aspects of normal rhythmicity influenced by this clock gene. Expand
Multiple ubiquitin mRNAs during xenopus laevis development contain tandem repeats of the 76 amino acid coding sequence
TLDR
It is shown that genomic DNA fragments exist that contain at least 12 of these units in tandem and proposed that the different mRNA size classes vary in their number of ubiquitin coding sequences. Expand
Circadian rhythm mutations in Drosophila melanogaster affect short-term fluctuations in the male's courtship song.
  • C. Kyriacou, J. C. Hall
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1980
TLDR
Both circadian rhythms and a very short, noncircadian oscillation appear to be influenced by the same gene. Expand
Nonessential Sequences, Genes, and the Polytene Chromosome Bands of DROSOPHILA MELANOGASTER.
TLDR
The discovery of these units, which are not allelic to any of the loci previously known, makes it clear that division 3B contains more genes than polytene chromosome bands, while portions of 3A and 3C seem to have no functional significance. Expand
P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic drosophila melanogaster
TLDR
Certain subsegments of the per region, transduced into the genome of arrhythmic pero flies, restore rhythmicity in circadian locomotor behavior and the male's courtship song. Expand
Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor
TLDR
A gene (MF alpha) coding for alpha-factor, a tridecapeptide mating factor secreted by yeast alpha cells, is cloned and sequenced and hypothesized to contain proteolytic processing signals. Expand
A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
A simple new procedure was described for producing a sequential series of overlapping clones for use in DNA sequencing. The technique used single-stranded M13 DNA and complementary DNA oligomers toExpand
Clock mutants of Drosophila melanogaster.
  • R. Konopka, S. Benzer
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1971
TLDR
Three mutants have been isolated in which the normal 24-hour rhythm is drastically changed and all these mutations appear to involve the same functional gene on the X chromosome. Expand
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