An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA

@article{Hirao2006AnUH,
  title={An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA},
  author={I. Hirao and M. Kimoto and T. Mitsui and T. Fujiwara and R. Kawai and A. Sato and Y. Harada and S. Yokoyama},
  journal={Nature Methods},
  year={2006},
  volume={3},
  pages={729-735}
}
  • I. Hirao, M. Kimoto, +5 authors S. Yokoyama
  • Published 2006
  • Biology, Medicine
  • Nature Methods
  • Methods for the site-specific incorporation of extra components into nucleic acids can be powerful tools for creating DNA and RNA molecules with increased functionality. We present an unnatural base pair system in which DNA containing an unnatural base pair can be amplified and function as a template for the site-specific incorporation of base analog substrates into RNA via transcription. The unnatural base pair is formed by specific hydrophobic shape complementation between the bases, but… CONTINUE READING
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