An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA

  title={An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA},
  author={Ichiro Hirao and Michiko Kimoto and Tsuneo Mitsui and Tsuyoshi Fujiwara and Rie Kawai and Akira Sato and Yoko Harada and Shigeyuki Yokoyama},
  journal={Nature Methods},
Methods for the site-specific incorporation of extra components into nucleic acids can be powerful tools for creating DNA and RNA molecules with increased functionality. We present an unnatural base pair system in which DNA containing an unnatural base pair can be amplified and function as a template for the site-specific incorporation of base analog substrates into RNA via transcription. The unnatural base pair is formed by specific hydrophobic shape complementation between the bases, but… 
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Unnatural base pair systems for DNA/RNA-based biotechnology.
  • I. Hirao
  • Biology, Chemistry
    Current opinion in chemical biology
  • 2006
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