An unnatural base pair for incorporating amino acid analogs into proteins

@article{Hirao2002AnUB,
  title={An unnatural base pair for incorporating amino acid analogs into proteins},
  author={I. Hirao and T. Ohtsuki and T. Fujiwara and T. Mitsui and T. Yokogawa and T. Okuni and H. Nakayama and Koji Takio and T. Yabuki and T. Kigawa and K. Kodama and K. Nishikawa and S. Yokoyama},
  journal={Nature Biotechnology},
  year={2002},
  volume={20},
  pages={177-182}
}
  • I. Hirao, T. Ohtsuki, +11 authors S. Yokoyama
  • Published 2002
  • Medicine, Biology
  • Nature Biotechnology
  • An unnatural base pair of 2-amino-6-(2-thienyl)purine (denoted by s) and pyridin-2-one (denoted by y) was developed to expand the genetic code. The ribonucleoside triphosphate of y was site-specifically incorporated into RNA, opposite s in a template, by T7 RNA polymerase. This transcription was coupled with translation in an Escherichia coli cell-free system. The yAG codon in the transcribed ras mRNA was recognized by the CUs anticodon of a yeast tyrosine transfer RNA (tRNA) variant, which had… CONTINUE READING
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