An unnatural base pair for incorporating amino acid analogs into proteins

@article{Hirao2002AnUB,
  title={An unnatural base pair for incorporating amino acid analogs into proteins},
  author={Ichiro Hirao and Takashi Ohtsuki and Tsuyoshi Fujiwara and Tsuneo Mitsui and Tomoko Yokogawa and Taeko Okuni and Hiroshi Nakayama and Koji Takio and Takashi Yabuki and Takanori Kigawa and Koichiro Kodama and Takashi Yokogawa and Kazuya Nishikawa and Shigeyuki Yokoyama},
  journal={Nature Biotechnology},
  year={2002},
  volume={20},
  pages={177-182}
}
An unnatural base pair of 2-amino-6-(2-thienyl)purine (denoted by s) and pyridin-2-one (denoted by y) was developed to expand the genetic code. The ribonucleoside triphosphate of y was site-specifically incorporated into RNA, opposite s in a template, by T7 RNA polymerase. This transcription was coupled with translation in an Escherichia coli cell-free system. The yAG codon in the transcribed ras mRNA was recognized by the CUs anticodon of a yeast tyrosine transfer RNA (tRNA) variant, which had… Expand
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