An ultrasensitive, nonisotopic immunoassay for hyaluronan using the streptavidin-biotin system.

@article{Rler1998AnUN,
  title={An ultrasensitive, nonisotopic immunoassay for hyaluronan using the streptavidin-biotin system.},
  author={Andreas R{\"o}\ssler},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1998},
  volume={270 2},
  pages={101-14}
}
  • Andreas Rößler
  • Published 1998 in
    Clinica chimica acta; international journal of…
A time-resolved fluoroimmunoassay for measuring hyaluronan concentrations in plasma and several biological fluids is described. The solid-phase immunoassay is based on the competition between aggregation of hyaluronan with the cartilage proteoglycan monomer, followed by binding of a monoclonal antibody to keratan sulfate of the proteoglycan and a biotinylated anti-mouse IgG. Fluorescence can be measured by a time-resolved fluorometer after binding of Eu(3+)-labelled streptavidin to the… CONTINUE READING