An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli

@inproceedings{Nehring2016AnUL,
  title={An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli},
  author={Ralf Bernd Nehring and Franklin Gu and Hsin-Yu Lin and Janet Lynn Gibson and Martin J. Blythe and Ray W Wilson and Mar{\'i}a Ang{\'e}lica Bravo N{\'u}{\~n}ez and P J Hastings and Edward J Louis and Ryan Lewis Frisch and James C. Hu and Susan M Rosenberg},
  booktitle={Nucleic acids research},
  year={2016}
}
With the wide availability of whole-genome sequencing (WGS), genetic mapping has become the rate-limiting step, inhibiting unbiased forward genetics in even the most tractable model organisms. We introduce a rapid deconvolution resource and method for untagged causative mutations after mutagenesis, screens, and WGS in Escherichia coli. We created Deconvoluter-ordered libraries with selectable insertions every 50 kb in the E. coli genome. The Deconvoluter method uses these for replacement of… CONTINUE READING

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