An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes

  title={An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes},
  author={Gabrielle Wheway and Miriam Schmidts and Dorus A Mans and Katarzyna M. Szymańska and Thanh-Minh T. Nguyen and Hilary Racher and Ian G. Phelps and Grischa Toedt and Julie E. Kennedy and Kirsten A. Wunderlich and Nasrin Sorusch and Zakia I.A. Abdelhamed and Subaashini Natarajan and Warren Herridge and Jeroen van Reeuwijk and Nicola Horn and Karsten Boldt and David A Parry and Stef J. F. Letteboer and Susanne Roosing and Matthew R. Adams and Sandra M. Bell and Jacquelyn Bond and Julie L Higgins and Ewan E. Morrison and Darren Charles Tomlinson and Gisela G G Slaats and Teunis J P van Dam and Lijia Huang and Kristin Kessler and Andreas Giessl and Clare V Logan and Evan A. Boyle and Jay Shendure and Shamsa Anazi and Mohammed A. Aldahmesh and Selwa A Al Hazzaa and Robert A Hegele and Carole Ober and Patrick Frosk and Aizeddin A. Mhanni and Bernard N. Chodirker and Albert E. Chudley and Ryan E Lamont and Francois P. Bernier and Chandree L. Beaulieu and Paul Gordon and Richard T. Pon and Clem Donahue and A. James Barkovich and Louis Wolf and Carmel Toomes and Christian T Thiel and Kym M. Boycott and Martin McKibbin and Chris F. Inglehearn and Fiona J. Stewart and Heymut Omran and Martijn A. Huynen and Panagiotis I. Sergouniotis and Fowzan S Alkuraya and Jillian S. Parboosingh and A Micheil Innes and Colin Eric Willoughby and Rachel H Giles and Andrew R. Webster and Marius Ueffing and Oliver E Blacque and Joseph G Gleeson and Uwe Wolfrum and Philip L. Beales and Toby J. Gibson and Daniel A Doherty and Hannah M. Mitchison and Ronald Roepman and Colin A. Johnson},
  booktitle={Nature Cell Biology},
Defects in primary cilium biogenesis underlie the ciliopathies, a growing group of genetic disorders. We describe a whole-genome siRNA-based reverse genetics screen for defects in biogenesis and/or maintenance of the primary cilium, obtaining a global resource. We identify 112 candidate ciliogenesis and ciliopathy genes, including 44 components of the… CONTINUE READING