An oxyferrous heme/protein-based radical intermediate is catalytically competent in the catalase reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG).

@article{Suarez2009AnOH,
  title={An oxyferrous heme/protein-based radical intermediate is catalytically competent in the catalase reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG).},
  author={Javier Martinez Suarez and Kalina Ranguelova and Andrzej A. Jarzecki and Julia Manzerova and Vladimir N Krymov and Xiangbo Zhao and Shengwei Yu and Leonid Metlitsky and Gary J. Gerfen and Richard S. Magliozzo},
  journal={The Journal of biological chemistry},
  year={2009},
  volume={284 11},
  pages={7017-29}
}
A mechanism accounting for the robust catalase activity in catalase-peroxidases (KatG) presents a new challenge in heme protein enzymology. In Mycobacterium tuberculosis, KatG is the sole catalase and is also responsible for peroxidative activation of isoniazid, an anti-tuberculosis pro-drug. Here, optical stopped-flow spectrophotometry, rapid freeze-quench EPR spectroscopy both at the X-band and at the D-band, and mutagenesis are used to identify catalase reaction intermediates in M… CONTINUE READING

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Catalase Activity in M. tuberculosis KatG 7028 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME

  • X. Zhao, S. Girotto, S. Yu, R. S. andMagliozzo
  • J. Biol. Chem. 279,
  • 2004

Bacterial Resistance to Antimicrobials: Mechanisms, Genetics, Medical Practice and Public Health (Wax

  • A. S. Pym, S. T. Cole
  • 2002

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