An oxidized derivative of cholesterol increases the release of soluble vascular cell adhesion molecule-1 from human umbilical vein endothelial cells in culture.

@article{Tamasawa2001AnOD,
  title={An oxidized derivative of cholesterol increases the release of soluble vascular cell adhesion molecule-1 from human umbilical vein endothelial cells in culture.},
  author={Naoki Tamasawa and Hitomi Murakami and Jun Y Matsui and Kazumi Yamato and G JingZhi and Tadaatsu Imaizumi and Kazuma Fujimoto and Hidemi Yoshida and Kei Satoh and Toshihiro Suda},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1531 3},
  pages={178-87}
}
Treatment of human umbilical vein endothelial cells (HUVECs) with 7-ketocholesterol resulted in an increased release of soluble vascular cell adhesion molecule-1 (VCAM-1) into culture medium. 7-Ketocholesterol did not enhance the expression of mRNA for VCAM-1. 7 beta-Hydroxy- or 25-hydroxycholesterol had no effect on soluble VCAM-1 levels. Western blot analysis revealed that soluble VCAM-1, in the conditioned medium of both 7-ketocholesterol-stimulated and control cells, had a molecular size of… CONTINUE READING