An overlay gel method for identification and isolation of bacterial beta-lactamases.

Abstract

A modification of the iodometric technique using an overlay gel was employed for fast identification and isolation of beta-lactamase types TEM, SHV and AmpC from non-denaturing gels. Osmotic shock preparations of the three beta-lactamases were run on polyacrylamide gels without SDS and ampicillin containing overlay gels were flooded with the iodine solution… (More)

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.