An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes.

@article{Italia2017AnOP,
  title={An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes.},
  author={James S Italia and Partha Sarathi Addy and Chester J.J. Wrobel and Lisa A. Crawford and Marc J. Lajoie and Yunan Zheng and Abhishek Chatterjee},
  journal={Nature chemical biology},
  year={2017},
  volume={13 4},
  pages={
          446-450
        }
}
In this study, we demonstrate the feasibility of expanding the genetic code of Escherichia coli using its own tryptophanyl-tRNA synthetase and tRNA (TrpRS-tRNATrp) pair. This was made possible by first functionally replacing this endogenous pair with an E. coli-optimized counterpart from Saccharomyces cerevisiae, and then reintroducing the liberated E. coli TrpRS-tRNATrp pair into the resulting strain as a nonsense suppressor, which was then followed by its directed evolution to genetically… CONTINUE READING
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