An orthogonal active site identification system (OASIS) for proteomic profiling of natural product biosynthesis.

@article{Meier2009AnOA,
  title={An orthogonal active site identification system (OASIS) for proteomic profiling of natural product biosynthesis.},
  author={Jordan L Meier and Sherry M Niessen and Heather S. Hoover and Timothy L. Foley and Benjamin F Cravatt and Michael D. Burkart},
  journal={ACS chemical biology},
  year={2009},
  volume={4 11},
  pages={948-57}
}
A significant gap exists between genetics-based investigations of polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) biosynthetic pathways and our understanding of their regulation, interaction, and activity in living systems. To help bridge this gap, here we present an orthogonal active site identification system (OASIS) for the proteomic identification and analysis of PKS/NRPS biosynthetic enzymes. OASIS probes target conserved features of PKS/NRPS active sites to provide… CONTINUE READING

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