An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae.

@article{Banerjee2006AnOD,
  title={An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae.},
  author={Sanjib Banerjee and Rukhsana Chowdhury},
  journal={Microbiology},
  year={2006},
  volume={152 Pt 4},
  pages={1055-62}
}
5-Methyl cytosine (m5C) was detected in genomic DNA of the enteric pathogen Vibrio cholerae by HPLC analysis and immunoblotting with m5C-specific antibody. Although cleavage with the restriction endonuclease EcoRII revealed the absence of a Dcm homologue in V. cholerae, analysis of the genome sequence indicated the presence of a gene, designated in this study as vchM, which encodes a DNA (cytosine-5-)-methyltransferase (m5C-MTase) designated M.Vch. M.Vch is not associated with a restriction… CONTINUE READING

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Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases.

Chembiochem : a European journal of chemical biology • 2002
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