An organ culture of postnatal rat liver slices

  title={An organ culture of postnatal rat liver slices},
  author={Adele Hart and Frederick J. Mattheyse and John B. Balinsky},
  journal={In Vitro},
SummaryA technique for the organ culture of postnatal and adult rat liver has been developed. Liver slices, 0.3 mm thick, were maintained in Conway units at the interphase between medium and a 95% O2:5% CO2 atmosphere. Postnatal liver in culture for up to 72 h had healthy hepatocytes throughout the explants; if adult liver was used the upper 0.2 mm was healthy after 24 h. These slices incorporated tritiated orotate and leucine into trichloroacetic acid-precipitable material. Incorporation of… Expand
Maintenance of adult rat liver slices in dynamic organ culture
This system provides a simple and effective method for the culture and biochemical maintenance of adult rat liver for 20 h with minimal loss of biochemical function. Expand
Histomorphological changes and cytochrome P450 isoforms expression and activities in precision-cut liver slices from neonatal rats.
Liver slices from neonatal rats are excellently suited for the evaluation of an in vitro induction of CYP enzymes as well, and even a maturation of the tissue occurred during the 24 h of incubation. Expand
Three-dimensional culture of hepatocytes on porcine liver tissue-derived extracellular matrix.
The culture of hepatocytes on 3D liver-specific ECM resulted in considerably improved cell growth and maintained cell function; therefore, this system could potentially be used in liver tissue regeneration, drug discovery or toxicology studies. Expand
Precision-cut liver slices from rats of different ages: basal cytochrome P450-dependent monooxygenase activities and inducibility
The biotransformation capacity – of the cytochrome P450 (CYP) system for example – is lower but inducibility is more pronounced in neonates than in adults. On the other hand, both enzyme activitiesExpand
Evaluation of fibrosis in precision-cut tissue slices
It is concluded that the early onset of fibrosis can be induced successfully in PCTS prepared from human and experimental animals and both healthy and fibrotic human tissue slices will pave the way for the testing of novel therapeutic drugs to treat patients with fibrosis avoiding interspecies extrapolation. Expand
Investigating the protective role of death receptor 3 (DR3) in renal injury using an organ culture model.
The OC model has proven to be an efficient tool for studying human cell heterogeneity, basal and regulated receptor expression, signalling pathways, and various biological responses not readily achievable in traditional cell culture models. Expand


Functional integrity of fetal rat liver explants cultured in a chemically defined medium.
This technique provides a simple method for maintaining viable fetal liver explants in a chemically defined medium, and should be useful for studying metabolic development in antenatal liver. Expand
Bile acid conjugation in fetal hepatic organ cultures.
The results indicate that metabolic processes in the organ-culture system are in a state of dynamic equilibrium and that morphologic integrity and specific hepatocytic function are maintained after 21 days in vitro. Expand
Maintenance of viable cells in an organ culture of mature rat liver.
Small pieces of liver from 5–6 week old rats have been studied in organ culture in a defined chemical medium and it proved impossible to maintain a viable culture in 3 atm O2. Expand
Insulin and hydrocortisone effects on viability and glycogen stores of postnatal rat liver organ culture.
Organ cultures prepared from 3- to 6-day-old newborn rat liver were maintained for as long as 6 days in medium CMRL-1066 supplemented with horse serum, glucose, and L-glutamine; the atmosphere forExpand
Cytoplasmic vacuolization in a small percentage of cells and potassium loss are the only indications of cell injury detected, and the isolated cells are comparable to normal hepatic parenchymal cells in situ in appearance and function. Expand
Induction of Tyrosine-α-Ketoglutarate Transaminase in Fetal Rat and Fetal Human Liver in Organ Culture
The hypothesis that mammalian liver is incompetent to respond to hormone-induced enzyme synthesis before a certain developmental stage is reached is supported. Expand
The culture of mature organs in a synthetic medium.
The survival of brain, liver, thymus, spleen, bone marrow, testis and pancreas was unsatisfactory and partial survival was obtained in the case of kidney, adrenal and spinal ganglion. Expand
Induction of tyrosine-alpha-ketoglutarate transaminase in fetal rat liver.
  • W. Wicks
  • Biology, Medicine
  • The Journal of biological chemistry
  • 1968
Tests against antibody showed that the tyrosine transaminases in fetal and adult liver are immunologically identical and proved that the increase in enzyme activity in explants exposed to hydrocortisone was due to an increase in enzymes protein. Expand
In vitro induction of tyrosine aminotransferase in liver slices by hydrocortisone.
Data indicate that glucocorticoid acts only to increase the rate of TA synthesis without affecting its rate of degradation, which is similar to that of the basal, noninduced system. Expand
Differentiation of hepatic and hematopoietic cells and synthesis of blood serum proteins in organ cultures of the liver.
Adult liver of mice treated by CCl 4 12–72 h before explantation acquired the capacity to grow in organ cultures and to secrete serum albumin in the culture medium, and this capacity seems to correlate with the degree of lymphoid infiltration of the liver tissue prior toexplantation. Expand