An organ culture of postnatal rat liver slices

@article{Hart2007AnOC,
  title={An organ culture of postnatal rat liver slices},
  author={Adele Hart and Frederick J. Mattheyse and John B. Balinsky},
  journal={In Vitro},
  year={2007},
  volume={19},
  pages={841-852}
}
SummaryA technique for the organ culture of postnatal and adult rat liver has been developed. Liver slices, 0.3 mm thick, were maintained in Conway units at the interphase between medium and a 95% O2:5% CO2 atmosphere. Postnatal liver in culture for up to 72 h had healthy hepatocytes throughout the explants; if adult liver was used the upper 0.2 mm was healthy after 24 h. These slices incorporated tritiated orotate and leucine into trichloroacetic acid-precipitable material. Incorporation of… Expand
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