An optimized xylene-free protein extraction method adapted to formalin-fixed paraffin embedded tissue sections for western blot analysis.

@article{Mansour2017AnOX,
  title={An optimized xylene-free protein extraction method adapted to formalin-fixed paraffin embedded tissue sections for western blot analysis.},
  author={A. Mansour and P. A. Khalil and Noha A. Bejjani and R. Chatila and Carole Dagher-Hamalian and Wissam H. Faour},
  journal={Histology and histopathology},
  year={2017},
  volume={32 3},
  pages={
          307-313
        }
}
Deparaffinization of formalin-fixed paraffin embedded (FFPE) tissues with xylene currently remains a major challenge to the biomedical community. [...] Key Method A total of 79 different types of FFPE tissue sections of 8 µm thickness were obtained from various archived FFPE specimens. Deparaffinization was conducted by gently washing each section with around 1 ml of hot distilled water (≈80°C).Expand
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