An optimized protocol to determine the engulfment of cancer cells by phagocytes using flow cytometry and fluorescence microscopy.

@article{Nam2019AnOP,
  title={An optimized protocol to determine the engulfment of cancer cells by phagocytes using flow cytometry and fluorescence microscopy.},
  author={Gi-hoon Nam and Yeonsun Hong and Yoon Jeong Choi and Gi Beom Kim and Yoon Kyoung Kim and Yoosoo Yang and In-San Kim},
  journal={Journal of immunological methods},
  year={2019},
  volume={470},
  pages={
          27-32
        }
}
The engulfment of cancer cells by macrophages is an important cellular process in innate cancer immunity. Antitumor immunotherapy that utilizes the enhanced engulfment of cancer cells by phagocytic cells has attracted much attention. Therefore, there is a growing demand for methods of measuring cancer cell phagocytosis. Quantifying the various stages of phagocytosis is invaluable for elucidating cancer-immune responses during this process. Here, we describe two phagocytosis assays, a flow… CONTINUE READING