An optimized protocol for the production of interdelta markers in Saccharomyces cerevisiae by using capillary electrophoresis.

@article{Tristezza2009AnOP,
  title={An optimized protocol for the production of interdelta markers in Saccharomyces cerevisiae by using capillary electrophoresis.},
  author={Mariana Tristezza and Carmela Gerardi and Antonio Francesco Logrieco and Francesco Grieco},
  journal={Journal of microbiological methods},
  year={2009},
  volume={78 3},
  pages={286-91}
}
The amplification of genomic sequence blocks flanked by delta elements of retrotransposon origin has proved to be a very convenient method for molecular characterization of Saccharomyces cerevisiae strains. Fluorescent automated capillary electrophoresis (CE) was used to detect interdelta marker (IDM) patterns in S. cerevisiae, using the ABI Prism 3130 Genetic Analyzer. Main experimental parameters were studied and the optimal conditions for IDM amplification and samples run on the CE apparatus… CONTINUE READING

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