An optimized procedure for the capture, fractionation and proteomic analysis of proteins using hydrogel nanoparticles.

@article{Rainczuk2010AnOP,
  title={An optimized procedure for the capture, fractionation and proteomic analysis of proteins using hydrogel nanoparticles.},
  author={Adam Rainczuk and Katie Meehan and David L. Steer and Peter Gordon Stanton and David M. Robertson and Andrew N Stephens},
  journal={Proteomics},
  year={2010},
  volume={10 2},
  pages={332-6}
}
We have developed an optimized procedure using dual size exclusion/affinity hydrogel nanoparticles to capture and comparatively analyze low molecular mass proteins directly from biological samples. The method described facilitates charge- and size-dependent protein binding, direct analysis by MS or other means and is highly reproducible. A comparative analysis of the low molecular mass proteome of plasma following freeze-thaw immediately after venipuncture is used to illustrate proof-of-concept… CONTINUE READING