An optimized preparation method to obtain high-quality RNA from dry sunflower seeds.

@article{Ma2011AnOP,
  title={An optimized preparation method to obtain high-quality RNA from dry sunflower seeds.},
  author={Xiao-na Ma and J Yang},
  journal={Genetics and molecular research : GMR},
  year={2011},
  volume={10 1},
  pages={160-8}
}
In an attempt to isolate high-quality, intact total RNA from sunflower (Helianthus annuus) seeds for investigation of the molecular mechanisms of mutations, we tested various procedures, using kits, including RNAiso Plus, RNAiso Plus+RNAiso-mate for Plant Tissue, Trizol, and the Qi method, but no high-quality total RNA of high integrity was obtained with any of these methods, probably due to the high content of polyphenols, polysaccharides, and secondary metabolites in mature sunflower seeds… CONTINUE READING
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