An optimized microplate assay system for quantitative evaluation of plant cell wall-degrading enzyme activity of fungal culture extracts.

@article{King2009AnOM,
  title={An optimized microplate assay system for quantitative evaluation of plant cell wall-degrading enzyme activity of fungal culture extracts.},
  author={Brian Christopher King and Marie K. Donnelly and Gary C Bergstrom and Larry P. Walker and Donna M. Gibson},
  journal={Biotechnology and bioengineering},
  year={2009},
  volume={102 4},
  pages={1033-44}
}
Developing enzyme cocktails for cellulosic biomass hydrolysis complementary to current cellulase systems is a critical step needed for economically viable biofuels production. Recent genomic analysis indicates that some plant pathogenic fungi are likely a largely untapped resource in which to prospect for novel hydrolytic enzymes for biomass conversion. In order to develop high throughput screening assays for enzyme bioprospecting, a standardized microplate assay was developed for rapid… CONTINUE READING

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