An optimized method for the generation of AFLP markers in a stingless bee (Melipona quadrifasciata) reveals a high degree of intracolonial genetic polymorphism

@inproceedings{Makert2006AnOM,
  title={An optimized method for the generation of AFLP markers in a stingless bee (Melipona quadrifasciata) reveals a high degree of intracolonial genetic polymorphism},
  author={Gustavo R. Makert and Robert J Paxton and Klaus Hartfelder},
  year={2006}
}
This study describes an optimized protocol for the generation of Amplified Fragment Length Polymorphism (AFLP) markers in a stingless bee. Essential modifications to standard protocols are a re- striction enzyme digestion (EcoRI and Tru1I) in a two-step procedure, combined with a touchdown program in the selective PCR amplification step and product labelling by incorporation of α( 33 P)dATP. In an analysis of 75 workers collected from three colonies of Melipona quadrifasciata we obtained 719… CONTINUE READING

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