An optimized method for intensive screening of molecules that stimulate beta-defensin 2 or 3 (hBD2 or hBD3) expression in cultured normal human keratinocytes.

@article{Pernet2005AnOM,
  title={An optimized method for intensive screening of molecules that stimulate beta-defensin 2 or 3 (hBD2 or hBD3) expression in cultured normal human keratinocytes.},
  author={Ingrid Pernet and Corinne Reymermier and Anne Guezennec and Jacqueline Viac and Jo{\"e}lle Guesnet and Eric Perrier},
  journal={International journal of cosmetic science},
  year={2005},
  volume={27 3},
  pages={
          161-70
        }
}
Normal human skin controls the intrusion of microorganisms by the production of peptide antibiotics such as defensins. The aim of our study was to develop a culture model of normal human keratinocytes for optimal beta-defensin mRNA detection which allows the screening of molecules able to stimulate hBD2 and hBD3 without inducing pro-inflammatory cytokines. A keratinocyte culture model in 96-well plates, in high calcium medium (1.7 mm) allowed to analyze hBD2 and hBD3 mRNA expression in basal… CONTINUE READING