An optimized approach to membrane capacitance estimation using dual-frequency excitation.

@article{Barnett1997AnOA,
  title={An optimized approach to membrane capacitance estimation using dual-frequency excitation.},
  author={David W. Barnett and Stanley Misler},
  journal={Biophysical journal},
  year={1997},
  volume={72 4},
  pages={1641-58}
}
We present an optimized solution to the problem of membrane impedance estimation when a patch-clamped cell is stimulated by a dual-frequency, sinusoidal excitation. The complete data set of raw whole-cell current samples is typically reduced, via digital lock-in detection, to measurements of the complex cell model admittance at the two stimulus frequencies. We describe a statistical model of both data sets and demonstrate that the admittance data adequately represent the essential features… CONTINUE READING